TransFect Protocol Database. and two new DNA transfection reagents: Attractene Transfection Reagent and NanoFect Transfection Reagent. I tried Lipofectamine and Attractene which both had a bad efficiency. plate format to do your experiment, the transfection protocol will be DNA/well. 年7月29日 QIAGEN Supplementary Protocol Protocol: Fast?Forward Transfection of cells with DNA using Attractene Transfection Reagent This.
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Rather than adapting existing protocols to fit the cell type, the TransFect Database enables researchers to access exactly the protocol needed by simply entering the cell type, nucleic acid, and plate format into the online system.
The optimal incubation time proyocol gene expression analysis depends on the cell type, the gene expressed, and the method of analysis.
For this reason, further optimization is recommended to achieve maximum transfection efficiency. Forward Transfection of cells with DNA using Attractene Transfection Reagent This protocol uses standard parameters and might require further optimization for transfection with this cell type. However, for some cell types, especially difficult-to-transfect cells such as suspension cells and primary cells, higher transfection efficiencies may be achieved by plating cells 24 hours prior to transfection for a protocol, consult the Attractene Transfection Reagent Handbook at www.
Using Attractene Reagent also ensures exceptionally low cytotoxicity. The instrument range of Hygiena International Ltd, experts in rapid microbial detection, monitoring…. The amount of transfection reagent and DNA required for optimal performance may vary, depending on the cell line and gene target. Routing Attrqctene vs Ro In this protocol, cell plating and transfection are performed on the same day. This reagent is suitable for a range of transfection applications, including transient or stable transfection, transfection of shRNA attracgene RNA vectors, wttractene DNA cotransfection.
This can be determined by performing a time? Simple Safe Parallel Reaction Sampling. As a starting prtoocol, we recommend using 6-well plate?
Home PCR Cell strategies for high tr Shortly before transfection, seed 3x cells per well of a 6-well plate in ? Use the reagent and nucleic acid amounts listed in the protocol. Use of the TransFect Database is free of charge and no registration is required. Maintain cells in selective culture medium until colonies appear. The Fast-Forward Protocol, in which cell plating and transfection are performed on the same day, has been shown to work successfully with a wide variety of cell types.
Prime thermal cyclers provide flexible PCR Added: Absence of animal-derived components also facilitates regulatory compliance. Change the medium as required. Transfection optimization Optimizing DNA 0.
The latest reagents use advanced technology to meet all DNA transfection needs.
Cell strategies for high transfection efficiency | Laboratory Talk
NanoFect Reagent is completely chemically synthesised, free attracteen animal-derived components, and has been tested for the absence of endotoxins. In addition, the absence of lipids makes this reagent suitable for lipid or signal transduction research. Ease of handling means that Attractene Reagent is suitable for use with automated systems.
To complement and enhance the existing portfolio protoclo trusted transfection solutions, Qiagen has launched the TransFect Protocol Database and two new DNA transfection reagents: For stable transfections, passage cells into the appropriate selection medium 24?
The table below shows a pipetting scheme with 5 different conditions we recommend to test when optimizing transfection i. As a general rule, optimization of transfection can be performed by varying DNA and Attractene Reagent amount as follows: Don’t have an account?
Cell strategies for high transfection efficiency
The amounts given are for one well of a 6-well plate. Incubate the samples for 10? Cells may alternatively be seeded after step 3 of this protocol.